Calibration of a 50 mL burette is prepared by filling the burette with distilled water, removing air bubbles from the head, and draining until less than 0.00 mL. The initial temperature of the water is recorded to determine the floating density of the water. After weighing a 50 ml Erlenmeyer flask with a rubber stopper, carefully avoiding fingerprints by worn gloves, the initial water level in the burette was recorded to the nearest 0.50 mL. The water in the burette is distributed in an Erlenmeyer flask pre-weighed about 10 mL at a time, while measurements of the burette volume and bottle are taken between each time period until 50 mL is distributed. The whole process was done a total of two times without completely drying before weighing between trials.